hello, I really want to know what caused this, looking forward to your reply
i used this code :
res <- BisqueRNA::ReferenceBasedDecomposition(bulk.eset, sc.eset, use.overlap=FALSE)
the error is:
Decomposing into 9 cell types.
Using 8000 genes in both bulk and single-cell expression.
Converting single-cell counts to CPM and filtering zero variance genes.
Filtered 0 zero variance genes.
Converting bulk counts to CPM and filtering unexpressed genes.
Error in CountsToCPM(bulk.eset) :Zero expression in selected genes for 1 cells
hello, I really want to know what caused this, looking forward to your reply
i used this code : res <- BisqueRNA::ReferenceBasedDecomposition(bulk.eset, sc.eset, use.overlap=FALSE)
the error is:
Decomposing into 9 cell types. Using 8000 genes in both bulk and single-cell expression. Converting single-cell counts to CPM and filtering zero variance genes. Filtered 0 zero variance genes. Converting bulk counts to CPM and filtering unexpressed genes. Error in CountsToCPM(bulk.eset) : Zero expression in selected genes for 1 cells