cpouchon / REFMAKER

Make your own nuclear references from genomic assemblies of shotgun libraries.
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degenerate base #6

Open Yolanda1201 opened 3 months ago

Yolanda1201 commented 3 months ago

hello, when I get the final matrix, I find lots of degenerate bases (R, K, Y, M...)in my matrix. I wonder if you meet the problem and how you handle it. should I just ignore the bases or delete them? And importantly, will they bias the result? Looking forward to your reply!