Closed xiaonalu closed 1 year ago
Hi @xiaonalu, yes you can, see https://github.com/crisprVerse/Tutorials/tree/master/Design_Custom_Sequence. The input sequence should be longer than the length of the Cas9 spacer sequence (20nt); some on-target algorithms require some sequence context around the spacer sequence, so it might return NA
values if the input sequence is too short.
Awesome!!! Thanks
Hi, I was wondering if I could use my interested sequence as input for the sgRNA design (cas9), on/off target score? If possible, is any length limitation for my input? Thanks