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crisprVerse / Tutorials

Tutorials for the crisprVerse
MIT License
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use interested sequence as input #1

Closed xiaonalu closed 1 year ago

xiaonalu commented 1 year ago

Hi, I was wondering if I could use my interested sequence as input for the sgRNA design (cas9), on/off target score? If possible, is any length limitation for my input? Thanks

Jfortin1 commented 1 year ago

Hi @xiaonalu, yes you can, see https://github.com/crisprVerse/Tutorials/tree/master/Design_Custom_Sequence. The input sequence should be longer than the length of the Cas9 spacer sequence (20nt); some on-target algorithms require some sequence context around the spacer sequence, so it might return NA values if the input sequence is too short.

xiaonalu commented 1 year ago

Awesome!!! Thanks