Hi, I encounter an error at the very last step during the design of sgRNA for CRISPR activation.
Everything is going fine until I try to add the CRISPRai scores.
snapshotDate(): 2023-10-24
see ?crisprScoreData and browseVignettes('crisprScoreData') for documentation
loading from cache
/bin/sh: RNAfold: command not found
Traceback (most recent call last):
File "/Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/library/crisprScore/python/crisprai/getWeissmanScores.py", line 105, in <module>
scores = predictWeissmanScore(tssTable, p1p2Table, sgrnaTable, libraryTable, pickleFile, fastaFile, chromatinFiles, modality, verbose)
File "/Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/library/crisprScore/python/crisprai/getWeissmanScores.py", line 26, in predictWeissmanScore
paramTable = getParamTable(tssTable, p1p2Table, sgrnaTable, libraryTable, fastaFile, chromatinFiles, verbose)
File "/Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/library/crisprScore/python/crisprai/getWeissmanScores.py", line 61, in getParamTable
raise Exception('Error generating parameter table.')
Exception: Error generating parameter table.
Erreur dans file(file, "rt") : impossible d'ouvrir la connexion
De plus : Message d'avis :
Dans file(file, "rt") :
impossible d'ouvrir le fichier '/var/folders/64/0s_gdgs13436hlvh44zjw1q00000gn/T//RtmpMYYf2X/scores.txt' : No such file or directory
Hi, I encounter an error at the very last step during the design of sgRNA for CRISPR activation. Everything is going fine until I try to add the CRISPRai scores.
After typing the command line in R
I get the following error message:
Here are my session info
Could you help me on this matter ? Best