Open abearab opened 1 year ago
Hi @abearab,
Yes, addSpacerAlignmentsIterative
should work on PairedGuideSet
objects, but it seems the data are too big to be handled for the underlying CompressedGRangesList
class from the GRanges package; I'll work on a fix, but in the meantime, I'd suggest to do some filtering on your individual gRNAs before pairing them to reduce the size of the object, or alternatively, split your PairedGuideSet object into a list of PairedGuideSet objects and apply the functional sequentially.
Got it, thank you for your response. I'll get back to you if I ran to another related issue.
Is it possible to run
addSpacerAlignmentsIterative
onPairedGuideSet
object?! I an error running this code:This is the size of my
pairsGuideSet
: