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crisprVerse / crisprDesign

Comprehensive design of CRISPR gRNAs for nucleases and base editors
MIT License
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DNA and RNA bulge off target DSB #23

Closed frvcw closed 12 months ago

frvcw commented 1 year ago

Hi

I want to consider the off target risk of DNA and gRNA bulges in the design of my protospacers. Is there a system in CrisprVerse that allows me to do it with my custom pakage genomes or should I rely on other programs e.g. Cas OFF finder?

Thank for your work

Jfortin1 commented 12 months ago

Hi @frvcw,

Unfortunately, our current aligners don't support DNA/RNA bulges