Closed AudreyDub closed 1 year ago
Hi @AudreyDub,
The on- and off-target scoring algorithms were developed for specific nucleases (enPAMGV was developed for the engineered Cas12a for instance), so addOnTargetScores
and addOffTargetScores
cannot be used for your custom nuclease.
Hi, Actually i use a custom nuclease sequence define like this:
How can i define a correct method for my custom nuclease?
At the end, the idea is to obtain a GuideSet with on/Off target :)
Thx Audrey