Error，‘Connection refuse’d and ‘550 Failed to change directory’ during step 13, please help me

[XingshengYang]

Hi all, I meet error “550 Failed to change directory” in step 13 as issue # 63 mentioned. I changed the link “ftp://ftp.ebi.ac.uk/pub/databases/interpro/current_release/interpro.xml.gz” according to the answer. But the connection was refused. Please give me some tips to resolve it. Thanks!!!

2023-04-17 07:49:12,400 [INFO]: This is MicrobeAnnotator v2.0.4 2023-04-17 07:49:12,401 [INFO]: I will download and format the databases I use. 2023-04-17 07:49:12,401 [INFO]: Creating database folders 2023-04-17 07:49:12,401 [INFO]: Step 13 2023-04-17 07:49:12,401 [INFO]: Creating RefSeq to UniProt table 2023-04-17 07:49:16,595 [INFO]: Finished 2023-04-17 07:49:16,618 [INFO]: Creating KO to EC table 100% [..............................................................................] 50126 / 501262023-04-17 07:49:18,063 [INFO]: Finished 2023-04-17 07:49:18,065 [INFO]: Creating InterPro tables Traceback (most recent call last): File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 1566, in ftp_open fw = self.connect_ftp(user, passwd, host, port, dirs, req.timeout) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 1588, in connect_ftp persistent=False) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 2408, in init self.init() File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 2417, in init self.ftp.connect(self.host, self.port, self.timeout) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/ftplib.py", line 154, in connect source_address=self.source_address) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/socket.py", line 728, in create_connection raise err File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/socket.py", line 716, in create_connection sock.connect(sa) ConnectionRefusedError: [Errno 111] Connection refused

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "/data/softwares/miniconda3/envs/microbeannotator/bin/microbeannotator_db_builder", line 445, in main() File "/data/softwares/miniconda3/envs/microbeannotator/bin/microbeannotator_db_builder", line 437, in main single_step, aspera, keep_temp, bin_path) File "/data/softwares/miniconda3/envs/microbeannotator/bin/microbeannotator_db_builder", line 285, in database_duilder database_directory, interconversion_database) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/site-packages/microbeannotator/database/conversion_database_creator.py", line 157, in create_interpro_tables wget.download("ftp://ftp.ebi.ac.uk/pub/databases/interpro/current_release/interpro.xml.gz", out=download_output) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/site-packages/wget.py", line 526, in download (tmpfile, headers) = ulib.urlretrieve(binurl, tmpfile, callback) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 247, in urlretrieve with contextlib.closing(urlopen(url, data)) as fp: File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 222, in urlopen return opener.open(url, data, timeout) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 525, in open response = self._open(req, data) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 543, in _open '_open', req) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 503, in _call_chain result = func(*args) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 1584, in ftp_open raise exc.with_traceback(sys.exc_info()[2]) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 1566, in ftp_open fw = self.connect_ftp(user, passwd, host, port, dirs, req.timeout) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 1588, in connect_ftp persistent=False) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 2408, in init self.init() File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/urllib/request.py", line 2417, in init self.ftp.connect(self.host, self.port, self.timeout) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/ftplib.py", line 154, in connect source_address=self.source_address) File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/socket.py", line 728, in create_connection raise err File "/data/softwares/miniconda3/envs/microbeannotator/lib/python3.7/socket.py", line 716, in create_connection sock.connect(sa) urllib.error.URLError: <urlopen error ftp error: ConnectionRefusedError(111, 'Connection refused')>

[xuechunxu]

I got the same error. How to fix it?

[volcanihpc]

The URL in _.../database/conversion_databasecreator.py has changed. Edit line 155, and put this URL instead: "https://ftp.ebi.ac.uk/pub/databases/interpro/current_release/interpro.xml.gz"