PoGo is a proteogenomic tool which allows mapping of peptides identified through mass spectrometry to a reference genome through provided gene annotation.
Hello, I tried emailing the address listed on the PoGo website but it was kicked back. Here is the email, in case you check here:
I am attempting to use the Sanger Institute tool, PoGo. I have had success using it both on my own files and on the GENCODE reference files when those files contain human data. However, I am currently trying to use PoGo with mouse data (just the GENCODE reference files and a list of peptides) to create BED files and have had no luck. I don't get any errors, but the output files are empty.
Do you or any of your colleagues have advice on how to proceed? This is the command I'm using (just so you can see I am specifying mouse—I also have tried the Taxon ID listed on the PoGo website).
Hello, I tried emailing the address listed on the PoGo website but it was kicked back. Here is the email, in case you check here:
I am attempting to use the Sanger Institute tool, PoGo. I have had success using it both on my own files and on the GENCODE reference files when those files contain human data. However, I am currently trying to use PoGo with mouse data (just the GENCODE reference files and a list of peptides) to create BED files and have had no luck. I don't get any errors, but the output files are empty.
Do you or any of your colleagues have advice on how to proceed? This is the command I'm using (just so you can see I am specifying mouse—I also have tried the Taxon ID listed on the PoGo website).
PoGo -fasta gencode.vM34.pc_translations.fa -gtf gencode.vM34.annotation.gtf -in all_peptide_POGO.txt -format BED -species Mouse