We can consider adding pseudoalignment approaches (Kallisto/Salmon) for comparison with aligners, but since these approaches rely on transcriptome it makes little sense to do a transcriptome-based read simulation followed by alignment to that transcriptome if competing aligners were to target the whole genome. Valid options include:
Input transcriptome - simulation - (pseudo)alignment to transcriptome target using all tools
Input real RNA-Seq, (pseudo)aligned to either transcriptome or the genome or both depending on the tool
We can consider adding pseudoalignment approaches (Kallisto/Salmon) for comparison with aligners, but since these approaches rely on transcriptome it makes little sense to do a transcriptome-based read simulation followed by alignment to that transcriptome if competing aligners were to target the whole genome. Valid options include: