ctSkennerton / crass

The CRISPR assembler
http://ctskennerton.github.io/crass
GNU General Public License v3.0
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ERROR: parseSeqFiles failed #102

Open fanlu-f opened 2 years ago

fanlu-f commented 2 years ago

Hi,

I try to run my data using crass, but there were errors in several data sets.

-I installed crass using conda.

I try to split the original fastq file into several small files and run them separately. That can solve the problem.

Therefore, I would like to ask whether it is possible to use this method to combine the results of all subsets as the results of the original sample.

ctSkennerton commented 2 years ago

You can. There is a small risk that some CRISPR will not be detected in the split files but they would be rare in the data.

On Apr 20, 2022, at 01:16, fanlu-f @.***> wrote:

 Hi,

I try to run my data using crass, but there were errors in several data sets.

-I installed crass using conda.

20/04/2022_03:43 -- CRisprASSembler (crass) -- Version: 1.0.1 5m 18s ERR WorkHorse.cpp : int WorkHorse::doWork(Vecstr) : 191: FATAL ERROR: parseSeqFiles failed I try to split the original fastq file into several small files and run them separately. That can solve the problem.

Therefore, I would like to ask whether it is possible to use this method to combine the results of all subsets as the results of the original sample.

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