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ctSkennerton / minced

Mining CRISPRs in Environmental Datasets
GNU General Public License v3.0
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identify the spacer sense strand #31

Closed YiweiZhu closed 4 years ago

YiweiZhu commented 4 years ago

Hi， I am using minced to look for CRISPR array in P. aeruginosa which contains frequently the I-F subtype. In this subtype, the CRISPR array at the upstream of Cas genes transcripts in the opposite direction. However, the spacers of the upstream arrays listed in minced results are still in the same direction of the Cas genes. Is it possible for minced to identify the sense strand of spacers depending on the CRISPR/Cas types? 图片

ctSkennerton commented 4 years ago

No, minced doesn’t detect the direction of arrays.

On May 22, 2020, at 1:32 AM, Yiwei Zhu notifications@github.com wrote:

 Hi， I am using minced to look for CRISPR array in P. aeruginosa which contains frequently the I-F subtype. In this subtype, the CRISPR array at the upstream of Cas genes transcripts in the opposite direction. However, the spacers of the upstream arrays listed in minced results are still in the same direction of the Cas genes. Is it possible for minced to identify the sense strand of spacers according to the CRISPR/Cas types?

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YiweiZhu commented 4 years ago

Thank you all the same!