cxzhu / Paired-Tag

Analysis of Paired-Tag datasets
MIT License
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GEO data #2

Closed ManuSetty closed 3 years ago

ManuSetty commented 3 years ago

Thanks for this great resource!

I have a question about the GEO submission metadata. Some of the samples are associated with multiple antibodies in the annotation - could you please provide information on how to map the reads onto the specific antibody?

An example screenshot: image

How do I figure out the antibody source of each read in these samples ?

cxzhu commented 3 years ago

Hi @ManuSetty, Thank you for your interest in our data! You can find the sample barcode annotation (tissue region, antibodies, rep#) in Extended Dat Fig. 2a, and the "Nuclei_metaData.xls" file associated with the RNA count matrix file in GEO.

ManuSetty commented 3 years ago

Thanks for the quick clarification @cxzhu !

ManuSetty commented 3 years ago

Another quick question: Can you please provide the prefix and sample correspondence used to generate the Cell_ID in the "Nuclei_metaData.xls". Thanks!

cxzhu commented 3 years ago

Hi @ManuSetty,

Here is the prefix list: Sub_Lib# Prefix
H3K4me1H3K27ac#01 01
H3K4me1H3K27ac#02 02
H3K4me1H3K27ac#03 03
H3K4me1H3K27ac#04 04
H3K4me1H3K27ac#05 05
H3K4me1H3K27ac#06 06
H3K4me1H3K27ac#07 07
H3K4me1H3K27ac#08 08
H3K4me1H3K27ac#09 09
H3K4me1H3K27ac#10 10
H3K4me1H3K27ac#11 11
H3K4me1H3K27ac#12 12
H3K4me1H3K27ac#13 13
H3K4me1H3K27ac#14 14
H3K4me1H3K27ac#15 15
H3K4me1H3K27ac#16 16
H3K27me3H3K9me3#01 21
H3K27me3H3K9me3#02 22
H3K27me3H3K9me3#03 23
H3K27me3H3K9me3#04 24
H3K27me3H3K9me3#05 25
H3K27me3H3K9me3#06 26
H3K27me3H3K9me3#07 27
H3K27me3H3K9me3#08 28
H3K27me3H3K9me3#09 29
H3K27me3H3K9me3#10 30
H3K27me3H3K9me3#11 31
H3K27me3H3K9me3#12 32
H3K4me3_#01 41
H3K4me3_#02 42
H3K4me3_#03 43
H3K4me3_#04 44
H3K4me3_#05 45
H3K4me3_#06 46
H3K4me3_#07 47
H3K4me3_#08 48
Paired-seq_#01 51
Paired-seq_#02 52
Paired-seq_#03 53
Paired-seq_#04 54
Paired-seq_#05 55
Paired-seq_#06 56
ManuSetty commented 3 years ago

Perfect - thanks @cxzhu !