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cxzhu / Paired-seq

Paired-seq
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SRR8980188 full barcoded reads low. #5

Closed macros29 closed 3 years ago

macros29 commented 3 years ago

Hi Chenxu,

I downloaded the dataset from SRA and run reachtools combine2 on R1 and R2, but the full barcoded reads were reported at only 0.02%. Is that normal?

Best, Zhen

cxzhu commented 3 years ago

Hi @macros29,

The SRR8980188 is a four-level barcoding library (Paired-seq V1), please use: https://github.com/cxzhu/Paired-seq/blob/master/proc_DNA.sh to process.

Best, Chenxu

macros29 commented 3 years ago

Ah, I was looking at the wrong pipeline. Thanks for the response!