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czbiohub-sf / GenoPrimer

Automated primer design for genotyping CRISPR edited cells
BSD 3-Clause "New" or "Revised" License
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Premature stop of relaxing criteria when it finds one primer pair #6

Open duopeng opened 1 year ago

duopeng commented 1 year ago

Example:

(1/1) Processing cutsite: Genome:ensembl_GRCh38_latest, Chr:8, cut_coordinate: 21919773 [Warning] No primers found, relaxing criteria, iteration=1 [Warning] No primers found, relaxing criteria, iteration=2 [Warning] No primers found, relaxing criteria, iteration=3 Listing all possible PCR products < 6000 bp 0_F chr=8 21919618-21919635 0_R chr=8 21919921-21919904 product_size = 303 (intended PCR product) finished in 0.05 min, processed 1 site(s), found 1 primer pair(s), outputted 1 primer pair(s), 0 cutcite(s) failed to yield primers

duopeng commented 1 year ago

should continue to relax criteria until three pairs of primers are found