run_species error

[luoqingqing777]

Hi! I download one selected species database, using code midas2 database --download --midasdb_name gtdb --midasdb_dir my_midasdb_Catellicoccus --species_list species_list.txt (only GTDB have the species i want ,and there is only one species number in species_list.txt ) After that , i wanna run SPECIES flow.But it appear 'Processing 64000 queries' constantly

1654588151.2: Single sample abundant species profiling in subcommand run_species with args 1654588151.2: { 1654588151.2: "subcommand": "run_species", 1654588151.2: "force": false, 1654588151.2: "debug": true, 1654588151.2: "zzz_worker_mode": false, 1654588151.2: "batch_branch": "master", 1654588151.2: "batch_memory": 378880, 1654588151.2: "batch_vcpus": 48, 1654588151.2: "batch_queue": "pairani", 1654588151.2: "batch_ecr_image": "pairani:latest", 1654588151.2: "midas_outdir": "midas2_output", 1654588151.2: "sample_name": "DYG1", 1654588151.2: "r1": "reads/DYG1.decon_1.fastq.gz", 1654588151.2: "r2": "reads/DYG1.decon_2.fastq.gz", 1654588151.2: "midasdb_name": "gtdb", 1654588151.2: "midasdb_dir": "my_midasdb_Catellicoccus", 1654588151.2: "word_size": 28, 1654588151.2: "aln_mapid": null, 1654588151.2: "aln_cov": 0.75, 1654588151.2: "marker_reads": 2, 1654588151.2: "marker_covered": 2, 1654588151.2: "max_reads": null, 1654588151.2: "num_cores": 8 1654588151.2: } 1654588151.2: Create OUTPUT directory for DYG1. 1654588151.2: 'rm -rf midas2_output/DYG1/species' 1654588151.2: 'mkdir -p midas2_output/DYG1/species' 1654588151.2: Create TEMP directory for DYG1. 1654588151.2: 'rm -rf midas2_output/DYG1/temp/species' 1654588151.2: 'mkdir -p midas2_output/DYG1/temp/species' 1654588151.3: MIDAS2::fetch_midasdb_files::start 1654588161.3: MIDAS2::fetch_midasdb_files::finish 1654588161.5: MIDAS2::map_reads_hsblastn::start [HS-BLASTN] Loading database. Loading /media/atm3/user02/MIDAS2.0/sample/my_midasdb_Catellicoccus/markers/phyeco/phyeco.fa.sequence, size = 0.4GB Loading /media/atm3/user02/MIDAS2.0/sample/my_midasdb_Catellicoccus/markers/phyeco/phyeco.fa.bwt, size = 0.8GB Loading /media/atm3/user02/MIDAS2.0/sample/my_midasdb_Catellicoccus/markers/phyeco/phyeco.fa.sa, size = 0.8GB [HS-BLASTN] done. Time elapsed: 1.70 secs.

[HS-BLASTN] Processing /dev/stdin. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries. Processing 64000 queries.