czbiohub-sf / shrimPy

shrimPy: Smart High-throughput Robust Imaging & Measurement in Python
BSD 3-Clause "New" or "Revised" License
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Z-drift and XY stabilization #103

Closed edyoshikun closed 7 months ago

edyoshikun commented 8 months ago

This PR has the code for:

The PR can be merged to registration_v2 or to main after registration_v2 is merged.

edyoshikun commented 8 months ago

@ieivanov @talonchandler this is ready for your input. I was using this small timelapse from the opencell, but feel free to pick a different timelapse. I did some cropping

mantis stabilize-timelapse -i /hpc/projects/comp.micro/mantis/2023_11_01_OpenCell_infection/1-recon/opencell_timelapse_7_phase.zarr/0/1/000001 -o ./test_stabilization/ -j 4 -c 0 -y -z -v -s 200 200

EDIT: mantis stabilize-timelapse -i /hpc/projects/comp.micro/mantis/2023_09_22_A549_0.52NA_illum/1-recon-phase/A549_MitoViewGreen_LysoTracker_W3_FOV5_1_phase.zarr/0/FOV0/0 -o ./test_stabilization/ -j 4 -c 0 -y -z -v -s 200 200

Couple of things to note that we can change and require your input:

talonchandler commented 8 months ago

Off-the-cuff thoughts based on out quick paired session:

edyoshikun commented 7 months ago

This is being replaced by #108