determine correct LS data sampling

czbiohub-sf / shrimPy

shrimPy: Smart High-throughput Robust Imaging & Measurement in Python

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determine correct LS data sampling #25

Closed ieivanov closed 1 year ago

ieivanov commented 1 year ago

We needed to determine the appropriate LS galvo sampling step. We currently sample in 150 - 300 nm steps over ~150 um range. Our plan is to perform Fourier ring correlation analysis on beads data in rawdata/mantis/2023_03_30_beads/beads_ip_0.72_4 to determine the appropriate scan step.

talonchandler commented 1 year ago

Fixed by #59.

See slides for details: https://docs.google.com/presentation/d/1Lomg5S_NtAtK2XWy3dk28hmPu8b8W_-l/edit?usp=drive_link&ouid=115117580081308410885&rtpof=true&sd=true

We decided that we're correctly sampling the scan dimension and oversampling the axial dimension ~3x.

ieivanov commented 1 year ago

Let me also update the LS galvo scan step and the LF mirror scan step in the example configs to match what we discusses. Sorry, I said I'll do that, but never got around to it. Working on it now.