czbiohub-sf / shrimPy

shrimPy: Smart High-throughput Robust Imaging & Measurement in Python
BSD 3-Clause "New" or "Revised" License
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Improve autofocus reengagement #74

Closed ieivanov closed 1 year ago

ieivanov commented 1 year ago

This PR improves the autofocus re-enragement rate when arriving at a new well from ~ 65% to ~100% without increasing the acquisition time. This PR has been tested and is ready to merge.

As an experimental detail, imaging over a long time period works better when using the 12500 cSt silicone oil. I observed that even the 1000 cSt we currently use drips over time to the point where there is not enough oil on the plate to engage with the objective.

fixes #31