heesch_strict_riboseq_contaminants File

damhof / hofman_et_al_2023_seq

Code for Hofman et al., BioRxiv 2023 May 6;2023.05.04.539399. doi: https://doi.org/10.1101/2023.05.04.539399

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Open danielrebib opened 5 months ago

danielrebib commented 5 months ago

Hello,

Would it be possible to share the heesch_strict_riboseq_contaminants file required to build the bowtie2 index?

I have tried to build my own using gffUtils, but for some reason my bowtie2 does not align any reads to it.

Additionally, I was wondering why remove with bowtie2, rather than aligning with STAR and then ignoring reads mapping to rRNA?

Thank you!