Closed ahwanpandey closed 5 years ago
Also, are there any other parameters you would recommend for WGS data to get cleaner segmentation? Most of my data is 40x Germline and 80x Tumor. I don't really want to loose small focal changes but just looking at the plot, it seems to be pretty noisy.
Thanks!
I loaded the pileup created by cnv_facets into the original FACETS R library and was successful in generating the circles in the spider plot. It's just cnv_facets not showing them for some reason.
Hi- thanks for reporting this and apologies for the trouble. Could you post the version of cnv_facets from:
cnv_facets.R -v
Also it would be great if you could send me the csv pileup by putting it on e.g. dropbox so I can have a look and see if I can reporduce the bug. (To anonimise the data you can drop the REF and ALT columns with gunzip -c my_pileup.csv.gz | cut -f 1,2,5,6,9,10 -d',' | gzip > my_pileup.x.csv.gz
).
The version of facets that is built in cnv_facets may not be the latest available. If that is the case I can rebuild cnv_facets with the latest one.
Hey Dario,
Thanks for the reply. Here is the version:
cnv_facets.R -v
0.12.1; facets=0.5.14
And the data is here:
https://www.dropbox.com/sh/xeou3ildyhiqcd6/AAADFy0tKwj_f1EkKAsj_mM8a?dl=0
Thanks!
I just manually updated FACETS:
cnv_facets.R -v
0.12.1; facets=0.6.0
Still the same issue, but the plot has a legend now.
Indeed, this was an embarrassing bug now fixed (apologies). I'm going to upload version 0.13.0 to bioconda and it should be available there in a day or two.
Hello,
Thanks for this tool!
I ran cnv_facets on some whole genome samples with your recommended values. None of the spider plots have circles in them.
I have attached all the plots generated.
Note: I have also run the tool with various combinations of cval [25 400] [25 1000] and [50 1000] and they all have the same problem.
AOCS-116-1-3.cval_25_400.nbhd-snp_500.cov.pdf AOCS-116-1-3.cval_25_400.nbhd-snp_500.spider.pdf
Thanks!