databio / pepatac

A modular, containerized pipeline for ATAC-seq data processing
http://pepatac.databio.org
BSD 2-Clause "Simplified" License
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Traceback error when running looper for tutorial data #245

Closed beginnertobioinformatics closed 1 year ago

beginnertobioinformatics commented 1 year ago

Hello,

I was going through the extended tutorial for PEPATAC to test out the pipeline with the tutorial data. However, when I get to the last step and try running looper run examples/tutorial/tutorial_refgenie.yaml I receive the following error:

Traceback (most recent call last):
  File "/home/usr/PEPATACTut_venv/bin/looper", line 8, in <module>
    sys.exit(main())
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/looper.py", line 1115, in main
    run(args, rerun=(args.command == "rerun"), **compute_kwargs)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/looper.py", line 501, in __call__
    curr_pl_fails = cndtr.add_sample(sample, rerun=rerun)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/conductor.py", line 474, in add_sample
    self.submit()
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/conductor.py", line 514, in submit
    script = self.write_script(self._pool, self._curr_size)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/conductor.py", line 734, in write_script
    namespaces = _exec_pre_submit(pl_iface, namespaces)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/looper/conductor.py", line 848, in _exec_pre_submit
    _update_namespaces(namespaces, func(namespaces))
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/refgenconf/populator.py", line 45, in looper_refgenie_populate
    rgc = refgenconf.RefGenConf(rgc_path)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/refgenconf/refgenconf.py", line 96, in __init__
    super(RefGenConf, self).__init__(
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/yacman/yacman.py", line 125, in __init__
    file_contents = load_yaml(filepath)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/yacman/yacman.py", line 438, in load_yaml
    return read_yaml_file(filepath)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/yacman/yacman.py", line 418, in read_yaml_file
    with open(filepath, "r") as f:
FileNotFoundError: [Errno 2] No such file or directory: '$REFGENIE'
Exception ignored in: <function YacAttMap.__del__ at 0x7f5078ecaef0>
Traceback (most recent call last):
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/yacman/yacman.py", line 159, in __del__
    if hasattr(self[IK], FILEPATH_KEY) and not getattr(self[IK], RO_KEY, True):
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/attmap/pathex_attmap.py", line 59, in __getitem__
    v = super(PathExAttMap, self).__getitem__(item)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/attmap/ordattmap.py", line 48, in __getitem__
    return AttMap.__getitem__(self, item)
  File "/home/usr/PEPATACTut_venv/lib/python3.10/site-packages/attmap/attmap.py", line 32, in __getitem__
    return self.__dict__[item]
KeyError: '__internal'

I first thought the error might have to do with the version of looper I had (1.4.0), but when I tried downgrading it, it didn't fix the issue. Also, for the other FileNotFound error for $REFGENIE, I did run the export command (export REFGENIE=/home/usr/PEPATACTut_venv/pepatac_tutorial/tools/refgenie_config.yaml) and confirmed that it had successfully run with echo $REFGENIE, but I still get this error. Are these errors related? Or tied to the fact that I'm running these commands in a virtual environment?

I'm very new to running command-line tools, python, etc., so any help or insight from the community is massively appreciated! Thank you!

beginnertobioinformatics commented 1 year ago

Hello, I tried running PEPATAC in a conda environment and got the same error initially, but when I downgraded looper to 1.3.1, it successfully passed the dry run but now this error appears--

Using default schema: /home/usr/pepatac/pipelines/pipestat_output_schema.yaml
Traceback (most recent call last):
  File "/home/usr/pepatac/pipelines/pepatac.py", line 2779, in <module>
    sys.exit(main())
  File "/home/usr/pepatac/pipelines/pepatac.py", line 683, in main
    report_message(pm, assets_file, message)
  File "/home/usr/pepatac/pipelines/pepatac.py", line 202, in report_message
    pm._safe_write_to_file(report_file, message)
AttributeError: 'PipelineManager' object has no attribute '_safe_write_to_file'

### Pipeline failed at:  (07-12 23:24:55) elapsed: 0.0 _TIME_

Total time: 0:00:01
Failure reason: Pipeline failure. See details above.
Error in atexit._run_exitfuncs:
Traceback (most recent call last):
  File "/home/usr/miniconda3/envs/pepatac/lib/python3.9/site-packages/pypiper/manager.py", line 2156, in _exit_handler
    self.fail_pipeline(Exception("Pipeline failure. See details above."))
  File "/home/usr/miniconda3/envs/pepatac/lib/python3.9/site-packages/pypiper/manager.py", line 2001, in fail_pipeline
    raise exc
Exception: Pipeline failure. See details above.

I'm thinking this is due to a package version I have that perhaps needs to be downgraded (assuming pypiper?), but I'm not sure what/what version?

beginnertobioinformatics commented 1 year ago

It turned out I had to downgrade piper to 0.12.3, the minimum version listed on the requirements text file. I still run into errors further downstream, but since I now don't think they're relevant to this original posting, I'll close this issue.

donaldcampbelljr commented 1 year ago

This is related to Pypiper (piper). Version v0.13.0 deprecated using _safe_write_to_file in favor of pipestat.report

nsheff commented 1 year ago

it would be nice if pypiper v0.13.0 would retain backwards compatibility, so that existing pipelines still work, while preferring the change to pipestat.report. What do you think about doing a bugfix release, pypiper 0.13.1, to restore backwards compatibility?