Open AMChalkie opened 1 year ago
Hi, Thank you very much for using the tool and reporting the bugs to us!
I will investigate the error this weekend. It would be great if you can provide me some ribo-minus RNA seq data in mouse (any - public data is good so that I can download it) so I can test directly on the mouse genome and annotations.
Regarding the dep data, I put one file with fdr2d parameters estimated from human datasets in the docker image. You can use this option to enable it: -dep Circall/Data/Circall_depdata_human.RData
Regarding generating the dep file for the mouse genome, it typically needs both ribo-minus RNA seq and its corresponding ribo-minus RNA-R enzyme treated RNA seq (this enzyme aims to remove linear RNA so we would yield the true positive - this assumption may not be perfect but is common for circRNA detection) circRNA). By comparing the 2 library results, fdr2d estimates parameters and thus can prioritize "true" circRNAs.
we also have the script to getfdr2d https://github.com/datngu/Circall/blob/master/Circall_v1.0.1/R/getFdr.R but it seems to be a bug in docker, I will revisit that and update to you!
Hope that this is useful!
Best wishes and have a nice weekend! Dat
Thanks :) I appreciate the effort.
Here is an example dataset but I'm not 100% sure it is what you need.
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83823
Best wishes Alistair
Hi,
A couple of questions:
Best wishes Alistair
tail /mnts/scratch/circrna_using_Circall_docker/output/sgMDA5_total_d3_rep3/sgMDA5_total_d3_rep3_Circall.log 3286 1 0.1255279 292.0 3289 1 0.1255279 1109.0 3298 1 0.1255279 742.0 3340 1 0.1255279 494.0 3346 1 0.1255279 518.0 3483 1 0.1255279 764.0 3539 1 0.1255279 1927.0
Done! Results are save in /mnts/scratch/circrna_using_Circall_docker/output/sgMDA5_total_d3_rep3/sgMDA5_total_d3_rep3_Circall_final.txtError: object 'PEres' not found Execution halted
head /mnts/scratch/circrna_using_Circall_docker/output/sgMDA5_total_d3_rep3/sgMDA5_total_d3_rep3_Circall_final.txt chr start end geneID circID junction_fragment_count junction_FPM median_circlen 6 40683939 40709120 ENSMUSG00000068587 64068393940709120 5 0.62763969564998 2685 8 33669153 33685702 ENSMUSG00000031584 83366915333685702 5 0.62763969564998 735