Amino acid sequence has too many "A"

[yikehuaili]

Dear ProteinMPNN team, Thank you very much for your great efforts.I want to know why the designed amino acid sequence has a long string of A(i.g. APGDAAAALAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA)It’s looks unusual. Thank you for your helps in advance

[dauparas]

Hello! This might happen if the model is uncertain about the prediction, or the input backbone is of low quality. You could try adding negative alanine bias.

[yikehuaili]

Thank you so much!