davidemms / OrthoFinder

Phylogenetic orthology inference for comparative genomics
https://davidemms.github.io/
GNU General Public License v3.0
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Orthofinder skip the alignment #688

Open shaodongyan opened 2 years ago

shaodongyan commented 2 years ago

OrthoFinder version 2.5.4 Copyright (C) 2014 David Emms

2022-04-07 09:18:23 : Starting OrthoFinder 2.5.4 24 thread(s) for highly parallel tasks (BLAST searches etc.) 1 thread(s) for OrthoFinder algorithm

Checking required programs are installed

Test can run "mafft /home/dell/zhenjun/all_4/OrthoFinder/Results_Apr05_1/WorkingDirectory//_dependencies_check/SimpleTest.fa" - ok Test can run "raxml" - ok

Analysing Orthogroups

2022-04-07 09:18:26 : Starting MSA/Trees Species tree: Using 201 orthogroups with minimum of 30.9% of species having single-copy genes in any orthogroup

Inferring multiple sequence alignments for species tree

2022-04-07 09:19:05 : Done 0 of 201 2022-04-07 09:35:13 : Done 100 of 201

Inferring remaining multiple sequence alignments and gene trees

2022-04-07 09:55:56 : Done 0 of 94419

I don't know why.But Orthofinder skip the "Inferring multiple sequence alignments for species tree" stage.Then Orthonfinder will report the error such as"OGxxxx.fa"doesn't exist. Please help me!

shaodongyan commented 2 years ago

Such as ERROR, input file does not exist: /home/dell/zhenjun/all_4/OrthoFinder/Results_Apr05_1/../Results_Apr07_8/WorkingDirectory/Alignments_ids/OG0000055.fa WARNING: Unknown caught unknown exception

davidemms commented 2 years ago

Hi

Could you post the complete output please and I'll have a look.

Best wishes David

shaodongyan commented 2 years ago

Hi Thank you,David. I solve this problem by run Orthofider on another HPC. But I meet another problem is that i can't remove a specie form specieslist.txt.

image

Such as these . But this speices is still in Orthofinder next run. Please help me , Thank you!

davidemms commented 2 years ago

Hi

If recommend using the example dataset to quickly test that you're doing the right thing to remove species and then do the same with your full dataset.

Best wishes David