The default setting of the plotTSNE function for perplexity is n.cells/5 - that would be very high for Drop-seq data sets of better quality with more than 1000 cells -- the original tSNE paper recommends perplexity values between 5 and 50. In my hands, perplexity values of 20-40 indeed work better for larger cell numbers. I'm fine with the default, but perhaps it should be mentioned in the documentation that depending on the number of cells this should perhaps be adjusted.
The default setting of the
plotTSNE
function for perplexity is n.cells/5 - that would be very high for Drop-seq data sets of better quality with more than 1000 cells -- the original tSNE paper recommends perplexity values between 5 and 50. In my hands, perplexity values of 20-40 indeed work better for larger cell numbers. I'm fine with the default, but perhaps it should be mentioned in the documentation that depending on the number of cells this should perhaps be adjusted.