Hi,
I encountered an error OSError: 'GSM2644945_Untreated-R1.100000_chr1.cool' is not an HDF5 file. when running the hicDifferentialTAD function with the test dataset in .cool format you showed in the readme file.
Can you help me solve the problem? Do I need to use h5 instead?
ERROR:hicexplorer.hicDifferentialTAD:'GSM2644945_Untreated-R1.100000_chr1.cool' is not an HDF5 file.Traceback (most recent call last):
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/hicmatrix/lib/cool.py", line 55, in load
cooler_file = cooler.Cooler(self.matrixFileName)
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/cooler/api.py", line 89, in __init__
self._refresh()
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/cooler/api.py", line 93, in _refresh
with open_hdf5(self.store, **self.open_kws) as h5:
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/contextlib.py", line 119, in __enter__
return next(self.gen)
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/cooler/util.py", line 528, in open_hdf5
fh = h5py.File(fp, mode, *args, **kwargs)
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/h5py/_hl/files.py", line 533, in __init__
fid = make_fid(name, mode, userblock_size, fapl, fcpl, swmr=swmr)
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/h5py/_hl/files.py", line 226, in make_fid
fid = h5f.open(name, flags, fapl=fapl)
File "h5py/_objects.pyx", line 54, in h5py._objects.with_phil.wrapper
File "h5py/_objects.pyx", line 55, in h5py._objects.with_phil.wrapper
File "h5py/h5f.pyx", line 106, in h5py.h5f.open
OSError: Unable to open file (file signature not found)
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/hicexplorer/hicDifferentialTAD.py", line 124, in computeDifferentialTADs
hic_matrix_target = hm.hiCMatrix(
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/hicmatrix/HiCMatrix.py", line 56, in __init__
matrixFileHandler_load = self.matrixFileHandler.load()
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/hicmatrix/lib/matrixFileHandler.py", line 51, in load
return self.matrixFile.load()
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/hicmatrix/lib/cool.py", line 65, in load
log.warning("The following nodes are available: {}".format(cooler.fileops.list_coolers(self.matrixFileName.split("::")[0])))
File "/pod/2/li-lab/Ziwei/Anaconda3/envs/hicexplorer/lib/python3.9/site-packages/cooler/fileops.py", line 189, in list_coolers
raise OSError("'{}' is not an HDF5 file.".format(filepath))
OSError: 'GSM2644945_Untreated-R1.100000_chr1.cool' is not an HDF5 file.
Hi, I encountered an error
OSError: 'GSM2644945_Untreated-R1.100000_chr1.cool' is not an HDF5 file.
when running thehicDifferentialTAD
function with the test dataset in.cool
format you showed in the readme file.Can you help me solve the problem? Do I need to use h5 instead?
Thank you so much for your help!
HiCExplorer ==3.7.2, python=3.9.3
The command I am using:
The full error I got: