unboundLocalError: local variable 'out_bam_file' referenced before assignment

[distilledchild]

Welcome to the HiCExplorer GitHub repository! Before opening the issue please check that the following requirements are met :

• [x] Search whether this issue (or a similar issue) has been solved before using the search tab above. Link the previous issue if appropriate below.

• [x] Paste your HiCExplorer version (hicInfo --version) and your python version (python --version) below.

• [x] Have you checked our documentation on hicexplorer.readthedocs.io?

• [x] Do you use conda to install HiCExplorer?

• [x] Do you use the latest HiCExplorer release? If not, please install it via a conda environment: conda create --name hicexplorer hicexplorer=3.6 python=3.8 -c bioconda -c conda-forge and activate the environment: conda activate hicexplorer. Retry your command. You can exit a conda environment via conda deactivate. To learn more about conda and environments, please consider the following documentation.

Retry your command, is it solved now? If not please continue with the following:

• [x] Paste the full HiCExplorer command that produces the issue below (ignore if you simply spotted the issue in the code/documentation).

• [x] Paste the output printed on screen from the command that produces the issue below (ignore if you simply spotted the issue in the code/documentation).

hicInfo 3.7.2 Python 3.10.6 command: hicBuildMatrix --samFiles smpl_R1.bam smpl_R2.bam \ --binSize 1000 5000 10000 50000 25000 50000 100000 250000 500000 1000000 2500000 \ --restrictionSequence GAATC GAGTC GATTC GACTC GATC CTAAG CTGAG CTTAG CTCAG TTAA \ --danglingSequence AAT AGT ATT ACT GATC TAA TGA TTA TCA TA --restrictionCutFile GAATC.bed GAGTC.bed GATTC.bed GACTC.bed GATC.bed CTAAG.bed CTGAG.bed CTTAG.bed CTCAG.bed TTAA.bed \ --threads 4 --inputBufferSize 400000 --outBam smpl_hicexplorer_hic.bam \ --outFileName smpl_hicexplorer_hic_matrix.h5 \ --chromosomeSizes chrom.sizes \ --genomeAssembly rn7chr.fa \ --doTestRun --doTestRunLines 1000000 \ --QCfolder hicQC/

After running, I got an error, Traceback (most recent call last): File "hicexplorer/bin/hicBuildMatrix", line 7, in main() File "hicexplorer/lib/python3.10/site-packages/hicexplorer/hicBuildMatrix.py", line 1400, in main out_bam_file.write(mate1) UnboundLocalError: local variable 'out_bam_file' referenced before assignment

And, it seems to be stuck. Could you help me to solve this error if it is stuck and errors please?

[distilledchild]

I ran it just /hicQuickQC command, and it doesn't cause any errors.