Thanks to the great tools provided by the deeptools development team.
I have obtained a file with the computeMatrix reference-point --referencePoint TSS command, but my TSS result is not very ideal (the input sample is more abundant than the TSS of the IP sample), so I only focus on the TSS distribution of the macs2 identified peak region, reading the official documentation computeMatrixOperations, I found that computeMatrixOperations subset can only filter the sample/strands/value information , So I did filter matrix by myself,I'm not sure if this is feasible, here's my script, I tested it worked and found that there was no error:
Thanks to the great tools provided by the deeptools development team.
I have obtained a file with the
computeMatrix reference-point --referencePoint TSS
command, but my TSS result is not very ideal (the input sample is more abundant than the TSS of the IP sample), so I only focus on the TSS distribution of the macs2 identified peak region, reading the official documentation computeMatrixOperations, I found thatcomputeMatrixOperations subset
can only filter the sample/strands/value information , So I did filter matrix by myself,I'm not sure if this is feasible, here's my script, I tested it worked and found that there was no error:matrix_TSS_2K.gz file :generated from
computeMatrix reference-point --referencePoint TSS
test_peaks.narrowPeak file :generated frommacs2
Tool version
I wonder if this is feasible,thanks