dellytools / delly

DELLY2: Structural variant discovery by integrated paired-end and split-read analysis
BSD 3-Clause "New" or "Revised" License
413 stars 136 forks source link

Delly call on WGS data #355

Closed Ceonham closed 8 months ago

Ceonham commented 8 months ago

Hi Tobias, I'm working on plant genomes and tried to use the germline analysis pipeline you gave to call SVs of 10× resequencing data of a panel of over 200 diverse samples. I first used delly call on each sample in parellel and conbined them to generate a sites.bcf using delly merge. However, when I used delly call to genotype all samples, the following error occured: Error: Delly genotyping requires local SV assembly (INFO/CONSENSUS) and breakpoint (INFO/CONSBP) introduced in delly v1.1.7!

Does the germline pipeline fit with my data? Or I have to use delly call to generate the final vcf with a single thread directly (I failed to configure the multi-threading mode so I installed delly via conda)? Looking forward to your reply. Thank you!

tobiasrausch commented 8 months ago

Sorry, this was a bug in delly v1.1.7. I released a new version today (v1.1.8) and now delly merge should keep INFO/CONSBP for genotyping.

Ceonham commented 8 months ago

Thank you Tobias. The new version solved the problem :)


----------原邮件信息---------- 发件人:"Tobias Rausch" @.> 收件人:"dellytools/delly" @.> 抄送人:"Ceonham" @.>, "Author" @.> 主题:Re: [dellytools/delly] Delly call on WGS data (Issue #355) 发送时间:2023-10-10 20:06:13

Sorry, this was a bug in delly v1.1.7. I released a new version today (v1.1.8) and now delly merge should keep INFO/CONSBP for genotyping.

-- Reply to this email directly or view it on GitHub: https://github.com/dellytools/delly/issues/355#issuecomment-1755240413 You are receiving this because you authored the thread.

Message ID: @.***>

tobiasrausch commented 8 months ago

👍