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deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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rsem calculate expression error #123

Open ssri28 opened 4 years ago

ssri28 commented 4 years ago

Hi Team,

i am facing error in rsem calculate expression for mouse reference

Error: "rsem-parse-alignments Mus_musculus.GRCm38.dna_sm.toplevel EP.temp/EP EP.stat/EP EP.temp/EP.bam 3 -tag XM" failed! Plase check if you provide correct parameters/options for the pipeline!

comman used : /home/ec2-user/data/RSEM/rsem-calculate-expression -p 75 --paired-end --bowtie2 --bowtie2-path /home/ec2-user/data/bowtie2-2.3.4.1-linux-x86_64/ EP_R1_paired.fastq.gz EP_R2_paired.fastq.gz /home/ec2-user/data/genome/mouse/Mus_musculus.GRCm38.dna_sm.toplevel EP

Kindly help me to solve the issue