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deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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IsoPct #154

Closed mhjiang97 closed 3 years ago

mhjiang97 commented 3 years ago

Hi,

I'd like to know how the column IsoPct in isoforms.results is calculated. Does it represent column FPKM in isoforms.results divided by the same column in genes.results?

Thank you, Minghao

cndewey commented 3 years ago

Hi Minghao,

It is the the relative abundance of the isoform (TPM) divided by the relative abundance of its gene (TPM). Within a sample, FPKM is proportional to TPM, so your interpretation is correct as well.

Best, Colin