deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
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The index file cannot generate. #160

Open chief-wang opened 3 years ago

chief-wang commented 3 years ago

Hi, everyone.

I was using "rsem-prepare-reference"

I tried both the gff3 file and the gtf file which is converted from the gff3 file.

In the gtf case, the process stopped at the first line "The GTF file might be corrupted! Stop and the line chr1 maker exon 18930 19283 . - . transcript_id "spo02923" spinach_gene_v1.gff3.gz

In the gff3 case, the process stopped at the line2"Line 2 does not have attribute parent: chr1 maker mRNA 18930 24583 . - . ID=Spo02923". The mRNA lines do not attribute Parent nor should have one. It is a little confusing and seems like a paradox. http://www.spinachbase.org/download the gff3 file is downloaded from the website above.

Thank you