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deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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RSEM calculate expression doesn't work #165

Open drumcoco opened 3 years ago

drumcoco commented 3 years ago

Hi,

I've set up the code to run the calculate expression. However it has been giving me all kinds of error message. I tried lots of things and I still don't know what is wrong.

here is my code

rsem-calculate-expression --star --star-path /path/to/STAR-2.7.8a/bin/Linux_x86_64 /path/to/star/genomeParameters.txt --star-gzipped-read-file /users/data/sample1_1.fastq.gz /users/data/sample1_2.fastq.gz --paired-end -p 2 /path/to/index/human38.n2g.idx.fa sample_name

thank you!

RamRS commented 3 years ago

all kinds of error message

That cannot help us diagnose what's going on. Please show us the exact error messages you see.