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deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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Issue with rsem-prepare-reference! #170

Open g-s-2018 opened 2 years ago

g-s-2018 commented 2 years ago

Hi,

I am trying to run rsem-prepare-reference with no luck. I am not sure how t fix this error.

rsem-prepare-reference --gff3 KT992094.1.gff --gff3-genes-as-transcripts --bowtie KT992094.1.fasta rsv-rsem rsem-gff3-to-gtf --make-genes-as-transcripts KT992094.1.gff rsv-rsem.gtf Line 25 does not have 9 fields:

"rsem-gff3-to-gtf --make-genes-as-transcripts KT992094.1.gff rsv-rsem.gtf" failed! Plase check if you provide correct parameters/options for the pipeline!

Thanks in advance,