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deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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No valid read to estimate the length distribution! #194

Open TdzBAS opened 1 year ago

TdzBAS commented 1 year ago

I get following error message, even after post-alignment quality control with STAR when i use rsem-calculate-expression:

image

my commands are :

rsem-calculate-expression --bam --no-bam-output -p 8 --paired-end ${filename} Simulation/ref/reference Simulation/data

get the same error when i use the paired fasta files directly.

anyone with an idea?

TdzBAS commented 1 year ago

UPDATE: my original input were fasta files and not fastq-files, therefore adding the flag --no-qualities resolved the error and the issue can be closed!