I run the rsem-calculate-expression, but fails with the following command:
rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge hoge.temp/hoge.bam 3 -tag XM
The SAM/BAM file declares more reference sequences (130527) than RSEM knows (66801)!
"rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge.temp/oge.bam 3 -tag XM" failed! Plase check if you provide correct parameters/options for the pipeline!
I would appreciate your guidance on how to deal with this problem.
Hi,
I run the rsem-calculate-expression, but fails with the following command:
rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge hoge.temp/hoge.bam 3 -tag XM The SAM/BAM file declares more reference sequences (130527) than RSEM knows (66801)! "rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge.temp/oge.bam 3 -tag XM" failed! Plase check if you provide correct parameters/options for the pipeline!
I would appreciate your guidance on how to deal with this problem.