Open ckr123tw opened 7 years ago
Hi, I was running the following command:
rsem-calculate-expression --paired-end --alignments Aligned.toTranscriptome.out.bam GCA_000001405.15_GRCh38_no_alt sample1
And an error message popped out: [W::bam_hdr_read] EOF marker is absent. The input is probably truncated.
I checked the integrity of Aligned.toTranscriptome.out.bam by samtools quickcheck, but it seemed intact. Should I worry about the error message?
RSEM version: 1.2.31 samtools version: 1.4 STAR version: 2.5.2b
There seems problems in Aligned.toTranscriptome.out.bam output by STAR when gff file is used.
Hi, I was running the following command:
rsem-calculate-expression --paired-end --alignments Aligned.toTranscriptome.out.bam GCA_000001405.15_GRCh38_no_alt sample1
And an error message popped out: [W::bam_hdr_read] EOF marker is absent. The input is probably truncated.
I checked the integrity of Aligned.toTranscriptome.out.bam by samtools quickcheck, but it seemed intact. Should I worry about the error message?
RSEM version: 1.2.31 samtools version: 1.4 STAR version: 2.5.2b