deweylab / RSEM

RSEM: accurate quantification of gene and isoform expression from RNA-Seq data
http://deweylab.biostat.wisc.edu/rsem/
GNU General Public License v3.0
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rsem-prepare-reference: The GTF file might be corrupted! #91

Open Lucas-Maciel opened 6 years ago

Lucas-Maciel commented 6 years ago

Hi, I have been trying to perform a RNASeq analyze but I found this problem.

rsem-prepare-reference --gtf file.gtf Schisto-genoma.fasta references rsem-extract-reference-transcripts references 0 agoravai.gtf None 0 Schisto-genoma.fasta The GTF file might be corrupted! Stop at line : Chr_1 VerjoLab exon 11159 11220 . + . gene_id "Smp_186980"; transcript_id "Smp_186980" "rsem-extract-reference-transcripts references 0 file.gtf None 0 Schisto-genoma.fasta" failed! Plase check if you provide correct parameters/options for the pipeline!

Any ideas of what is wrong? Thank you