Holes in Eukaryotic Q pathways

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Holes remain in the queuine/queuosine pathways in eukaryotic organisms. While QTRT1 and QTRT2 are relatively well understood in their roles across many model eukaryotes, other biochemistries are recognized as being necessary for both the transport (cellular uptake, transport across mitochondrial membranes) and salvage of Q/q but have yet to be assigned enzymes thought to perform these functions.

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Google AI results re. Q/q salvage in eukaryotes:

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Several genes are annotated in PomBase with the phenotype ontology term: FYPO:0003480

Mutants ani1 (CENP-A amino terminus domain (NTD) isomerase Ani1) kgd1 (2-oxoglutarate dehydrogenase (lipoamide) (e1 component of oxoglutarate dehydrogenase complex)) mas5 (Hsp40 family DNAJ domain protein Mas5) mmb1 (mitochondrial microtubule binder Mmb1) qng1 (tRNA queuosine nucleoside glycosylase Qng1)

Of these, only two are noted as not having identified orthologs in budding yeast:

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Mug164 (mmb1) in UniProtKB: O74779

KEGG entry: spo:SPBC25B2.07c GFIT

Gene Clusters

• SPBC25B2.01
• SPBC25B2.02c(K05658)
• SPBC25B2.03
• SPBC25B2.04c(K19828)
• SPBC25B2.05(K06961)
• SPBC25B2.06c

Gene Cluster Annotations

• SPBC25B2.01 putative elongation factor 1 alpha-like protein (GFIT: HBS1-like)

• SPBC25B2.02c M-factor transporter Mam1 (GFIT: ABCB1)

• SPBC25B2.03 zf-C3HC4 type zinc finger (GFIT: NA)

• SPBC25B2.04c putative GTPase Mtg1 (GFIT: MTG1)

• SPBC25B2.05 rRNA-processing protein Mis3 (GFIT: KRR1)

• SPBC25B2.06c BTB/POZ domain protein Btb2 (GFIT: BTBD13-like)