DCC uses output from the STAR read mapper to systematically detect back-splice junctions in next-generation sequencing data. DCC applies a series of filters and integrates data across replicate sets to arrive at a precise list of circRNA candidates.
Hello,
I would like to use DCC on TCGA samples using the bam files available on GDC Data Portal. Unfortunately, fastq files are not avaiable (even with full authorized access to data), the only bam files available are generated using STAR 2 and are:
The data are paired, so perhaps I can use samtools to retrieve the fastq from the bam, however I don't know which of the bam files available is better for that use!
Thanks for any help you can address
Pietro
Hello, I would like to use DCC on TCGA samples using the bam files available on GDC Data Portal. Unfortunately, fastq files are not avaiable (even with full authorized access to data), the only bam files available are generated using STAR 2 and are:
The data are paired, so perhaps I can use samtools to retrieve the fastq from the bam, however I don't know which of the bam files available is better for that use! Thanks for any help you can address Pietro