DCC uses output from the STAR read mapper to systematically detect back-splice junctions in next-generation sequencing data. DCC applies a series of filters and integrates data across replicate sets to arrive at a precise list of circRNA candidates.
DCC 0.4.2 started
8 CPU cores available, using 2
WARNING: non-stranded data, the strand of circRNAs guessed from the strand of host genes
started circRNA detection from file 2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction
=> locating circRNAs (unstranded mode) [2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction]
=> sorting circRNAs (unstranded mode) [2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction]
finished circRNA detection from file 2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction
Combining individual circRNA read counts
Traceback (most recent call last):
File "/usr/local/bin/DCC", line 9, in
load_entry_point('DCC==0.4.2', 'console_scripts', 'DCC')()
File "build/bdist.linux-x86_64/egg/DCC/main.py", line 413, in main
UnboundLocalError: local variable 'CircSkipfiles' referenced before assignment
DCC -N -D 2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction
DCC 0.4.2 started 8 CPU cores available, using 2 WARNING: non-stranded data, the strand of circRNAs guessed from the strand of host genes started circRNA detection from file 2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction => locating circRNAs (unstranded mode) [2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction] => sorting circRNAs (unstranded mode) [2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction] finished circRNA detection from file 2M_WT_18C/2M_WT_18C_STAR_outputChimeric.out.junction Combining individual circRNA read counts Traceback (most recent call last): File "/usr/local/bin/DCC", line 9, in
load_entry_point('DCC==0.4.2', 'console_scripts', 'DCC')()
File "build/bdist.linux-x86_64/egg/DCC/main.py", line 413, in main
UnboundLocalError: local variable 'CircSkipfiles' referenced before assignment