DCC uses output from the STAR read mapper to systematically detect back-splice junctions in next-generation sequencing data. DCC applies a series of filters and integrates data across replicate sets to arrive at a precise list of circRNA candidates.
The problem arises when old pysam versions (in this case 0.7.4) are used. Not only fails the BAM file sorting check, but also the linear files will end up empty. The issue has already been fixed in commit 3fa54ac7b03c164124ee8212d2ca6f1681d52db6 by adding specific version requirements to the DCC setup file.
The problem arises when old pysam versions (in this case 0.7.4) are used. Not only fails the BAM file sorting check, but also the linear files will end up empty. The issue has already been fixed in commit 3fa54ac7b03c164124ee8212d2ca6f1681d52db6 by adding specific version requirements to the DCC setup file.
This issue is only for later reference.