stop at Using files _tmp_DCC/tmp_circCount and _tmp_DCC/tmp_coordinates for filtering

[ChrisLou-bioinfo]

2018-07-02 04:22:50,597 Combining individual circRNA read counts 2018-07-02 19:54:58,365 Swapping strand information 2018-07-02 19:56:08,607 Write in annotation 2018-07-02 19:56:08,607 Select gene features in Annotation file 2018-07-02 20:17:05,993 Filtering started 2018-07-02 20:17:05,993 Using files _tmp_DCC/tmp_circCount and _tmp_DCC/tmp_coordinates for filtering

process stop at 2018-07-02 20:17:05,993 Using files _tmp_DCC/tmp_circCount and _tmp_DCC/tmp_coordinates for filtering

run commend： DCC @samplesheet -mt1 @mate1 -mt2 @mate2 -D -R /home/dell/refer/hg38_DCC_Repeats.gtf -an /home/dell/refer/gencode.v28.primary_assembly.annotation.gtf -Pi -ss -L 20 -F -M -Nr 1 2 -fg -T 20

the monitor had showed nothing about DCC program

thanks

[tjakobi]

Dear @ChrisLou-bioinfo ,

does DCC exit with an error message (a Python error or similar on the console) or with a clean status 0? You may want to run DCC again with the -k flag in order to keep temporary files that may be helpful for debugging.

Cheers, Tobias

[ChrisLou-bioinfo]

Hi,Tobias Thanks for your reply There are some warnings about memory-error

Using files _tmp_DCC/tmp_circCount and _tmp_DCC/tmp_coordinates for filtering Traceback (most recent call last): File "/home/dell/.local/bin/DCC", line 11, in load_entry_point('DCC==0.4.6', 'console_scripts', 'DCC')() File "build/bdist.linux-x86_64/egg/DCC/main.py", line 339, in main File "build/bdist.linux-x86_64/egg/DCC/circFilter.py", line 60, in readcirc MemoryError

My Server Memory is 64GB Sample Number is 168

thanks

Chris

[tjakobi]

Dear @ChrisLou-bioinfo,

thank you for your feedback. I never ran DCC with so many samples, it may actually be a memory issue. I will see if I can reduce the memory usage in this part of the code.

Cheers, Tobias

[ChrisLou-bioinfo]

Thanks! Waiting for your software updated