HI:
I use the jacusa2 to run my amplicon data, but the result lost the last position of the ref, that is, if my ref is 182bp long, the jacusa2 result only print the 1-181bp. The parameters I used are listed below.
#############
java -jar jacusa2.jar call-1 -A \
-r call1.out \
-q 30 \
-c 10 \
-R ref.fa \
aligned.bam
HI: I use the jacusa2 to run my amplicon data, but the result lost the last position of the ref, that is, if my ref is 182bp long, the jacusa2 result only print the 1-181bp. The parameters I used are listed below. ############# java -jar jacusa2.jar call-1 -A \ -r call1.out \ -q 30 \ -c 10 \ -R ref.fa \ aligned.bam