Open Chan-W00 opened 2 months ago
What a fast... thanks for reply.
So, according to your answer, "chr1 14521 14522 call-2 0.0639599068288419 . 1,0,15,0 0,0,12,0 1,0,13,0 1,0,21,0 G" means that in chromosome number 1 loci 14521-2, condition_1 rep_1 has 1,0,15,0 base conversion from G to A,C,G,T, respectively.
I got it and this is reason why the third position (G) is higher than others.
I really want to caculate total editing number per gene but have no idea about it, yet. Is there any recommandation for me to do this?
Dear JACUSA2 team,
I am a truely new to bioinformatics and have been trying to analyze C-to-T base editing that is mediated APOBEC1 enzyme directly conjugated to protein of interest (POI).
My situation is:
java -jar JACUSA_v2.0.4.jar call-2 -r result.out cond.1_rep.1.bam,cond.1_rep.2.bam cond.2_rep.1.bam,cond.2_rep.2.bam -p 4
contig start end name score strand bases11 bases12 bases21 bases22 info filter ref
chr1 14521 14522 call-2 0.0639599068288419 . 1,0,15,0 0,0,12,0 1,0,13,0 1,0,21,0 G chr1 14522 14523 call-2 0.46276221056984923 . 0,16,0,0 0,12,0,0 0,15,0,0 0,21,0,1 C chr1 14541 14542 call-2 0.12227137530251753 . 17,0,2,0 13,0,1,0 11,0,4,0 23,0,1,0 A chr1 14573 14574 call-2 0.20814993206096233 . 24,0,8,0 22,0,1,0 26,0,5,0 25,0,2,0 A
My questions are:
Please.. Give me any answer, otherwise my professor will kill me 😹.