Unable to open: No such file or directory (2) Unable to open: No such file or directory (2) Error in if (fsize > 2147483647) { : 需要TRUE/FALSE值的地方不可以用缺少值

[Baozi9]

Hi， When I just run the plot function , it come with the following problem? Can anyone help me to deal it? thanks a lot！！！！！！！！！

plots <- plotData(cytotrace2_result = cytotrace2_result,

• annotation = annotation,
• is_seurat = TRUE) Preparing input for visualization. Performing log-normalization 0% 10 20 30 40 50 60 70 80 90 100% [----|----|----|----|----|----|----|----|----|----| **| Calculating gene variances 0% 10 20 30 40 50 60 70 80 90 100% [----|----|----|----|----|----|----|----|----|----| **| Calculating feature variances of standardized and clipped values 0% 10 20 30 40 50 60 70 80 90 100% [----|----|----|----|----|----|----|----|----|----| **| |=========================================================| 100% Unable to open: No such file or directory (2) Unable to open: No such file or directory (2) Error in if (fsize > 2147483647) { : 需要TRUE/FALSE值的地方不可以用缺少值

[savagyan00]

Hi,

Thank you for using CytoTRACE 2 and reaching out with this issue.

To better assist you, could you please confirm a few details about the steps leading up to this error?

• How was the cytotrace2() function called? Please provide the exact command used, if possible.
• If cytotrace2_result is a Seurat object, could you please confirm that the expression data is accessible from the RNA assay and that your annotation input is a data.frame object?

If possible, sharing the input data that generates this error could help us diagnose the issue more effectively. You can send the details to cytotrace2team@gmail.com.

We appreciate your cooperation!

[savagyan00]

Hello,

We're closing this issue due to inactivity, but please feel free to reach out if you continue to experience the problem or have any other questions.

We're here to help!