Closed tomkinsc closed 7 years ago
This has been refactored a bit. While the aforementioned command still exists, the same report can now be created by specifying the --commonBarcodes
argument to illumina_demux
(it's the path of the file to write with the barcode counts).
Thanks @tomkinsc for the headsup
Note to self: Update resource tarball to next tag release.. 1.5.6 (?) and test with --commonBarcodes <barcode_report_fn>
argument
Note to self: function included in upstream v1.6.0 release
Addressed by #30
A new sub-command,
common_barcodes
, has been added toviral-ngs/illumina.py
. This command writes a report listing the barcodes present in a sequencer run, sorted by frequency in descending order. The information is helpful for troubleshooting whether or not a barcode may have been entered incorrectly in the original sample sheet.This command will be available in the next release of viral-ngs.
It would be helpful to add a call to this command to the demultiplexing applet, so the barcode report is among those generated by that step.