dongzhang0725 / PhyloSuite

PhyloSuite is an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies
GNU General Public License v3.0
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Gene order display option for custom extraction #30

Closed biomguler closed 2 years ago

biomguler commented 3 years ago

Hi, I have a problem with analyzing the mitogenome of 20 amoeba species. Because I have multiple issues, I listed them below.

  1. The species have different genome structures than animal mitogenomes, mitogenomes contain fusion cox1 and cox2, rps, and rpl genes. I am trying to extract genes. When I choose differently than the "mitogenome" option, the gene order display screen is disappearing. So, I can't modify options. It results in losing some gene data in the itol gene order file (only contain default listed genes). Also, the "geneStat.cvs" file doesn't show these genes. How can I customize the gene list without losing the results; that I get when using the "mitogenome" extraction parameter?

  2. I am also trying to get RSCU plots. I can draw graf when I use a single mitogenome, but not with multiple. When I run the R script I get the error below.
    Error: Aesthetics must be either length 1 or the same as the data (62): x and fill

Note: I get information from the paper; that mentions mapping gene orders onto the tree and other features that I want to get only for the mitogenome extraction option. I am trying to find alternative solutions.

dongzhang0725 commented 2 years ago

I am working on a trial version of PhyloSuite, it contains your required functions, if you are interested, I can send it to you.

biomguler commented 2 years ago

Dear Zhang, It would be very nice to get an updated version of your software.


Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Mon, Oct 25, 2021 at 2:34 PM zhang dong @.***> wrote:

I am working on a trial version of PhyloSuite, it contains your required functions, if you are interested, I can send it to you.

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/dongzhang0725/PhyloSuite/issues/30#issuecomment-950879091, or unsubscribe https://github.com/notifications/unsubscribe-auth/ASTDFJ3XNP6GKQA4JBDHWODUIVFERANCNFSM5AAOMPWQ . Triage notifications on the go with GitHub Mobile for iOS https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675 or Android https://play.google.com/store/apps/details?id=com.github.android&referrer=utm_campaign%3Dnotification-email%26utm_medium%3Demail%26utm_source%3Dgithub.

dongzhang0725 commented 2 years ago

What system do you use? Win 10?

biomguler commented 2 years ago

Yes, I am using win 10.

On Tue, Oct 26, 2021, 08:22 zhang dong @.***> wrote:

What system do you use? Win 10?

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dongzhang0725 commented 2 years ago

Ok, I will send a packaged version to you later. There are some minor places need fine adjustment.

biomguler commented 2 years ago

Great, thanks!

On Tue, Oct 26, 2021, 08:26 zhang dong @.***> wrote:

Ok, I will send a packaged version to you later. There are some minor places need fine adjustment.

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dongzhang0725 commented 2 years ago

Could you send me your email address?

biomguler commented 2 years ago

Sure, here it is. @.***

On Tue, Oct 26, 2021, 10:00 zhang dong @.***> wrote:

Could you send me your email address?

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dongzhang0725 commented 2 years ago

image seems your email address was masked?

biomguler commented 2 years ago

I don't know why i will try to solve if you still unable to send.

On Tue, Oct 26, 2021, 10:22 zhang dong @.***> wrote:

[image: image] https://user-images.githubusercontent.com/15956921/138837126-d60ebf8f-ff68-47c3-a0d1-6f41acfe7919.png seems your email address was masked?

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dongzhang0725 commented 2 years ago

maybe you can send me a screenshot of your email

biomguler commented 2 years ago

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/dongzhang0725/PhyloSuite/issues/30#issuecomment-951707151, or unsubscribe https://github.com/notifications/unsubscribe-auth/ASTDFJ5BM7ELPZX4SWU2DHDUIZRIBANCNFSM5AAOMPWQ . Triage notifications on the go with GitHub Mobile for iOS https://apps.apple.com/app/apple-store/id1477376905?ct=notification-email&mt=8&pt=524675 or Android https://play.google.com/store/apps/details?id=com.github.android&referrer=utm_campaign%3Dnotification-email%26utm_medium%3Demail%26utm_source%3Dgithub.

dongzhang0725 commented 2 years ago

Dear Murat,

The attachment file is the trial version of PhyloSuite, which contains several functions you want:

  1. it can customize the gene list you want to extract via "Add row" button in "Genes" tab of extracter function.
  2. it can draw RSCU figure directly without R, however, I suspect that your RSCU problem might be caused by a bad input file, so I am not sure this new function will resolve your problem. If the problem persists, you may send your data to me to debug the problem..
  3. I added a "Draw gene order" function under Mitogenome menu, it takes "linear_order.txt" file (available in "files" folder of the results) as input to draw gene order figure. Please note that you need to extract the data anew to generate that file using this new version, as the format of this file has been changed.

By the way, this version of PhyloSuite is still under development (codonW function is not finished), so it may contain bugs. Please check your results carefully and contact me if you encounter a problem .

Sincerely,

Dong PhyloSuite_v1.2.2_Win_beta.rar https://drive.google.com/file/d/1Sthsi0LJKWytVM8nozor5gE80Mn8-UA7/view?usp=drive_web

bio-mguler @.***> 于2021年10月26日周二 下午4:40写道:

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub < https://github.com/dongzhang0725/PhyloSuite/issues/30#issuecomment-951707151 , or unsubscribe < https://github.com/notifications/unsubscribe-auth/ASTDFJ5BM7ELPZX4SWU2DHDUIZRIBANCNFSM5AAOMPWQ

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biomguler commented 2 years ago

Dear Dong, Thank you for the software. I need accession permission.

Best regards. Murat.

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 12:47 PM zhang dong @.***> wrote:

Dear Murat,

The attachment file is the trial version of PhyloSuite, which contains several functions you want:

  1. it can customize the gene list you want to extract via "Add row" button in "Genes" tab of extracter function.
  2. it can draw RSCU figure directly without R, however, I suspect that your RSCU problem might be caused by a bad input file, so I am not sure this new function will resolve your problem. If the problem persists, you may send your data to me to debug the problem..
  3. I added a "Draw gene order" function under Mitogenome menu, it takes "linear_order.txt" file (available in "files" folder of the results) as input to draw gene order figure. Please note that you need to extract the data anew to generate that file using this new version, as the format of this file has been changed.

By the way, this version of PhyloSuite is still under development (codonW function is not finished), so it may contain bugs. Please check your results carefully and contact me if you encounter a problem .

Sincerely,

Dong PhyloSuite_v1.2.2_Win_beta.rar < https://drive.google.com/file/d/1Sthsi0LJKWytVM8nozor5gE80Mn8-UA7/view?usp=drive_web

bio-mguler @.***> 于2021年10月26日周二 下午4:40写道:

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <

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dongzhang0725 commented 2 years ago

I authorized, did you access to it?

bio-mguler @.***> 于2021年10月26日周二 下午7:13写道:

Dear Dong, Thank you for the software. I need accession permission.

Best regards. Murat.

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 12:47 PM zhang dong @.***> wrote:

Dear Murat,

The attachment file is the trial version of PhyloSuite, which contains several functions you want:

  1. it can customize the gene list you want to extract via "Add row" button in "Genes" tab of extracter function.
  2. it can draw RSCU figure directly without R, however, I suspect that your RSCU problem might be caused by a bad input file, so I am not sure this new function will resolve your problem. If the problem persists, you may send your data to me to debug the problem..
  3. I added a "Draw gene order" function under Mitogenome menu, it takes "linear_order.txt" file (available in "files" folder of the results) as input to draw gene order figure. Please note that you need to extract the data anew to generate that file using this new version, as the format of this file has been changed.

By the way, this version of PhyloSuite is still under development (codonW function is not finished), so it may contain bugs. Please check your results carefully and contact me if you encounter a problem .

Sincerely,

Dong PhyloSuite_v1.2.2_Win_beta.rar <

https://drive.google.com/file/d/1Sthsi0LJKWytVM8nozor5gE80Mn8-UA7/view?usp=drive_web

bio-mguler @.***> 于2021年10月26日周二 下午4:40写道:

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <

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biomguler commented 2 years ago

Yes, but I have a problem opening rar file. [image: image.png]

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 1:52 PM zhang dong @.***> wrote:

I authorized, did you access to it?

bio-mguler @.***> 于2021年10月26日周二 下午7:13写道:

Dear Dong, Thank you for the software. I need accession permission.

Best regards. Murat.

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 12:47 PM zhang dong @.***> wrote:

Dear Murat,

The attachment file is the trial version of PhyloSuite, which contains several functions you want:

  1. it can customize the gene list you want to extract via "Add row" button in "Genes" tab of extracter function.
  2. it can draw RSCU figure directly without R, however, I suspect that your RSCU problem might be caused by a bad input file, so I am not sure this new function will resolve your problem. If the problem persists, you may send your data to me to debug the problem..
  3. I added a "Draw gene order" function under Mitogenome menu, it takes "linear_order.txt" file (available in "files" folder of the results) as input to draw gene order figure. Please note that you need to extract the data anew to generate that file using this new version, as the format of this file has been changed.

By the way, this version of PhyloSuite is still under development (codonW function is not finished), so it may contain bugs. Please check your results carefully and contact me if you encounter a problem .

Sincerely,

Dong PhyloSuite_v1.2.2_Win_beta.rar <

https://drive.google.com/file/d/1Sthsi0LJKWytVM8nozor5gE80Mn8-UA7/view?usp=drive_web

bio-mguler @.***> 于2021年10月26日周二 下午4:40写道:

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <

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dongzhang0725 commented 2 years ago

use the software "WinRAR" to unpack it.

bio-mguler @.***> 于2021年10月26日周二 下午7:56写道:

Yes, but I have a problem opening rar file. [image: image.png]

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 1:52 PM zhang dong @.***> wrote:

I authorized, did you access to it?

bio-mguler @.***> 于2021年10月26日周二 下午7:13写道:

Dear Dong, Thank you for the software. I need accession permission.

Best regards. Murat.

Murat Güler


Ph.D. Student | Genomic Epidemiology Group (C055) German Cancer Research Center | Deutsches Krebsforschungszentrum (DKFZ) Im Neuenheimer Feld 280, 69120 Heidelberg, Germany Phone: +49 1575 227 04 51 http://www.dkfz.de/en/genomische-epidemiologie/

On Tue, Oct 26, 2021 at 12:47 PM zhang dong @.***> wrote:

Dear Murat,

The attachment file is the trial version of PhyloSuite, which contains several functions you want:

  1. it can customize the gene list you want to extract via "Add row" button in "Genes" tab of extracter function.
  2. it can draw RSCU figure directly without R, however, I suspect that your RSCU problem might be caused by a bad input file, so I am not sure this new function will resolve your problem. If the problem persists, you may send your data to me to debug the problem..
  3. I added a "Draw gene order" function under Mitogenome menu, it takes "linear_order.txt" file (available in "files" folder of the results) as input to draw gene order figure. Please note that you need to extract the data anew to generate that file using this new version, as the format of this file has been changed.

By the way, this version of PhyloSuite is still under development (codonW function is not finished), so it may contain bugs. Please check your results carefully and contact me if you encounter a problem .

Sincerely,

Dong PhyloSuite_v1.2.2_Win_beta.rar <

https://drive.google.com/file/d/1Sthsi0LJKWytVM8nozor5gE80Mn8-UA7/view?usp=drive_web

bio-mguler @.***> 于2021年10月26日周二 下午4:40写道:

Sure, here it is.

On Tue, Oct 26, 2021, 10:29 zhang dong @.***> wrote:

maybe you can send me a screenshot of your email

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