Error- botocore.exceptions.NoRegionError: You must specify a region.

[ritututeja]

Describe the bug botocore.exceptions.NoRegionError: You must specify a region.

To Reproduce Steps to reproduce the behavior:

1. Dockerized seqc for integrating as an app in CyVerse Discovery Environment.
2. docker run --rm reetututeja/seqc:0.21 SEQC index -h
3. Also tried with the sample dataset- docker run --rm reetututeja/seqc:0.21 SEQC index --organism homo_sapiens --ensemble-release 93 --valid-biotypes protein_coding lincRNA antisense IG_V_gene IG_D_gene IG_J_gene IG_C_gene TR_V_gene TR_D_gene TR_J_gene TR_C_gene --read-length 101 --folder index --local

Expected behavior I am trying seqc with the sample dataset provided on this github repo

Screenshots

Assay

• 10x v2/v3, Drop-seq, In-drop, ...

Runtime Environment

• SEQC Version [e.g. v0.2.2]
• HPC/AWS/GCP/Desktop

Additional context Add any other context about the problem here.

[hisplan]

Hi,

Sorry for the late reply. You can find the pre-built dockerized SEQC here: https://github.com/dpeerlab/seqc-docker

About the error you mentioned: Even though SEQC can run locally using the --local parameter, SEQC was actually designed to run on AWS, thus you see that error message NoRegionError: You must specify a region even though you're trying to run it locally. The trick is that you can just set any AWS region of your choice to ignore that message. For example:

export AWS_DEFAULT_REGION=us-east-1

For Docker, you can do something like this:

docker run -it --rm -e AWS_DEFAULT_REGION=us-east-1 hisplan/seqc:0.2.7

I hope that helps. Closing this. Feel free to reopen if you still have issues.

Thanks!